Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	2,197,266	T→G	T170P (ACC→CCC)	yqhD	iron‑containing alcohol dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	2,197,266	0	T→G	100.0%	27.6	41	T170P (ACC→CCC)	yqhD	iron‑containing alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (31/0):  ref base (0/10):  total (31/10)

GGCGTCATTATTTTTCGAACGGTCAAAATATTGCTCGATCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTACCTGCTGCAACTTGTGCCGCATTATCCCGCGA  .  NC_013198/2197135‑2197408                                                                                                                                     |                                                                                                                                                      ggCGTCATTAATTTTCGAACGTTCAAAATATTGCTCGACCAATTGACTAAGCATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTAccc                                                                                                                                  <  1:279975/151‑1              ttgtaaCGGTCAAAATATTGCTCGATCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.ggg                                                                                                                   <  2:501020/147‑1              ttCGAACGGTCAAAATATTGCTCGATCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.ggg                                                                                                                   <  2:549899/151‑1                                      aTCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGGGGGTT.C.GGGCCGGGGG.ACCCAA.CCTTTCAT.GGGGTTTCCGGTTTGAAAATCACgggc                                                                                           >  2:247661/1‑149                                      aTCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.CGGCCAGGGGTACCCAA.CTTTTTAT.GGG.TTGCCGGGTTTGAAAACAcagggc                                                                                          >  2:505679/1‑146                                             gACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCAGTGGGACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAAACACGGAGCCGC.CGtt                                                                                  >  1:96778/1‑151                                             gACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGGCAGGGGGACCCCA.CTTTTTAT.GGG.GTGCCGGGTTTGAAAACAAGGAGCCGC.TGtt                                                                                  >  1:31694/1‑151                                             gACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGCCCGGGGGACCCAA.CTTTTCAT.GGG.TTGCCGGGTTAGAAAACACCGAGcccccgtt                                                                                   >  1:499102/1‑145                                              aCTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTc                                                                                 <  1:152738/151‑1                                               cTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGGCCGGGGGGCCCCA.CTTTTTAT.TGG.GTGCCGGGTTTGAAAACACGGAGCCGC.TGTTct                                                                                >  2:666123/1‑150                                                   ggATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTc                                                                            <  1:147199/151‑1                                                       gTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCCGTGGGTCCCCA.ATTTTCATTGGG.TGGCCGG.TTAGAAATGAAGGAGGCCC.TGTTCCTTTcccac                                                                        >  2:17796/1‑147                                                         ccATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCCGGGGGACCCCA.CTTTTCAT.TGG.TTGCCGGATTTGAAATCACGGAGCCGC.CGTTCATTTTACTAcc                                                                      >  1:419754/1‑151                                                         ccATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.CTGGGCCGGCGAACCCCAC.TTTTTTAA.GGG.TTCCGGGGTTCTAAAT.ACGCAGCCGC.ttctcgttttcccatc                                                                      >  1:685448/1‑136                                                              gacccAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTg                                                                 <  2:788116/148‑1                                                              gAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGCCCGGGGGACCCCA.CTTTTCAT.TGG.GTGGCGGGTTAGAAATCACCGAGGCGC.TGGTCATTTTCCCACCTGTTg                                                                 >  2:551965/1‑151                                                                                  tACTGGGACAGTATAGGTCAAGCTCGGGTCAAGGAACGAAATGGCGGGGG.GATT.T.GGGGCCGTGGGACCCAA.CTTTTTAT.GGG.GTTCCGGGTTTGAAATAACCGGGGCGC.TGTTCTTTTCATTACCTGGTG.GCGCCAAAGCTTAAatatta                                            >  1:862838/1‑149                                                                                  tACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGCCAGTGGGACCCAA.CTTTTTAT.GGG.GTGCCGGGTTTGAAAACAAGGAGCCGC.TGTTCATTTTTCTACCTGTTG.CCGCCAAAGTTTAAATATca                                            >  1:830853/1‑151                                                                                    cTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCCGGGGGACCCAA.ATTTTCAT.GGG.GTGCCGGGGTAGAAAACACGGAGGCGC.TGGTCATTTCATTACCTGTTG.CCGCCAACGTTAAAATATtaac                                          >  2:30422/1‑151                                                                                       ggACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GGTT.T.GGGGCAGCGGGACCCAA.CTTTTTAT.GGG.GTGCCGGGTTAGAAAAAACGGAGGAGC.TGTTTATTTTACTACCTGTTT.CCGCCAACGGTAAAAAATaaacaaa                                       >  2:824490/1‑150                                                                                       ggACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCCGGGGGACCCAA.CATTTTAT.TGG.TTGTCGGATTAGAAAACACCGAGACGC.TGTTCCTTTTACTCCCTGTTG.CCCCCCAAGTTAAAAAAAcaacaaa                                       >  2:34023/1‑150                                                                                       ggACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCAGGGGGACCCAA.CTTTTTAT.GGG.TTGCCGGGTTAGAAAAAACGGGGGCGC.CGTTCATTTTACTACCCGTTT.GCGCCAAAGTTAAAAAATCcacaac                                       >  1:352241/1‑151                                                                                       ggACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCCGTGGGACCCCA.CTTTTTAT.GGG.TTGCCGGGTTAGAAAACACGGAGGCGC.TGTTCATTTCACTACCTGTTT.CCGCCAACGTTAAAAAATCaacaac                                       >  1:675084/1‑151                                                                                       ggACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCAGGGGGACCCAA.CTTTTTAT.GGG.GTGCCGGGTTAGAAATCACGGAGGCGC.TGTTCATTTTACTACCCGTTG.CCGCCAACGTTAAAATATCaaaaac                                       >  1:834177/1‑151                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATTTC.GGGCC.GAGGGAGCCAACCTTTTCTT.GGG.T.GCCGGATTATGAAACAAGGAGCCGC.CGCTTGTTTTACTACCTGTTT.ACCCCAACATTTAAAAATCAAAAAACGGTa                                  >  1:418133/1‑151                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCCGGGGGACCCAA.CTTTTCAT.GGG.GTGGCGGGTTAGAAAACACGGGGGCGC.TGGTCATTTTACTACCTGTTT.CCGCCCAAGGTAAAataatcacaaaccgta                                  >  2:851915/1‑138                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGGCAGGGGGACCCAA.CCTTTCAT.GGG.TTGCCGGGTTAGAAAACACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATAACAACAACCggga                                  >  2:853812/1‑149                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCCGGGGGACCCAA.CTTTTCAT.GGG.TTGCCGGGTTAGAAATCACGGAGCCGC.CGTTCATTTTACTACCTGTTT.CCGCCACCGTTAAAAAATCAAAAAACggga                                  >  1:839592/1‑149                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCAGTGGGACCCAA.CTTTTTAT.GGG.GTGGCGGGGTAGAAAACACGGAGCCGC.CGTTCCTTTTACTACCTGTTG.CCGCCAACATTAAAATATaaacaaaccgga                                  >  1:263158/1‑145                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.T.GGGCCAGGGGGACCCCA.CTTTTCAT.GGG.TTGGCGGGTTAGAAATCAAGGAGCCGC.TGTTCATTTTACTACCCGTTG.CCGCCCACGTTAAAAAATTAACAAcccgga                                  >  1:244109/1‑147                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGCCAGGGGGACCCAA.CTTTTTAT.TGG.TTGCCGGATTAGAAAACACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATcaacaaaccgga                                  >  1:88878/1‑145                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GATT.C.GGGCCAGGGGGACCCAA.CTTTTCAT.GGG.TTGCCGGGTTAGAAAAAAAGGAGCCGC.TGTTCCTTTCCCTACCTGTTG.CCGCCCACGTTAAAATATCAACAACCggga                                  >  1:696688/1‑149                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GAGTCC.GGGCCGGGGGACCCCAC.CTTTT.AC.GGG.TTGCCGGGGTAGAAAAAAACGAGCCCC.CCGTCCTTTTACTTCCTGTTG.CCCCCCAAATTAAAATATcaaaaaaccgca                                  >  2:557771/1‑142                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GAGT.C.GGGGCAGGGGTGCCCAA.CATTTTAT.GGG.GTGCCGGGTTAGGAAACAACGAGCCGC.CGTTCATTTCACTACCCGTTG.GCCGCAACATTAAAAAAACACCAAcccgtc                                  >  2:537392/1‑147                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GAAT.C.GGGGCCGTGGGACCCAA.CTTTTCAT.GGG.GTGGCCGGTTTGAAAACAACGAACCGC.CCTTCATTTTACTACCCGTTG.CCCCCCACCCTAAAATATCAAAAACCGGTa                                  >  1:270767/1‑151                                                                                            gTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.GAAT.C.GGGGCCATGGGACCCCA.CCTTTCAT.GGG.GTGCCGGGGTTGAAAACACGGAGCCGC.CGTTCCTTTTTCTACCTCTTGTCCCCCAAAGTAaaaaaaaaaaaaacggtc                                   >  2:151492/1‑137                                                                                                      aGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTACCTGCTGCaa                        <  1:532253/151‑1                                                                                                                  gAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTACCTGCTGCAACTTGTGCCGCAt            <  2:705534/151‑1                                                                                                                  gAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTACCTGCTGCAACTTGTGCCGCAt            <  1:766884/151‑1                                                                                                                           tGGCGGGGG.GATT.C.CGGCCCGGGGGACCCCA.CTTTTTAT.GGG.TTGCCGGGTTTGAAAACACGGAGCCGCCGGTTCTTTTTCCT.CCTGTTT.CCCCCAACCGTAAAATATCAACAAACCGCAACCCCTGCAACTTGGTCCGCCTTATcccccc   >  2:197488/1‑148                                                                                                                            ggCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTTCCTGCTGCAACTTGTGCCGCATTATCCCGCGa  <  1:55615/151‑1                                                                                                                                     |                                                                                                                                                      GGCGTCATTATTTTTCGAACGGTCAAAATATTGCTCGATCAAATGACTAAGGATGTCCATTGAACCAGCCGCCGTTTGCCGTACTGGGACAGTATAGGTCAAGCTCGGGTCAAGAAACGAAATGGCGGGGG.TATT.C.GGGCCAGTGGTACCCAA.CTTTTCAT.GGG.TTGCCGGATTAGAAATCACGGAGCCGC.TGTTCATTTCACTACCTGTTG.CCGCCAACGTTAAAATATCAACAACCGGTACCTGCTGCAACTTGTGCCGCATTATCCCGCGA  .  NC_013198/2197135‑2197408 Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 7 ≤  ATCG  < 10 ≤  ATCG  < 40 ≤  ATCG