Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,091	2 bp→CA	coding (713‑714/795 nt)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,091	0	T→C	100.0%	67.6	17	S238S (TCA→TCG)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/9):  ref base (0/0):  total (8/9)
*	NC_013198	1,873,092	0	G→A	100.0%	65.7	17	S238L (TCA→TTA)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/9):  ref base (0/0):  total (8/9)

GACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCG  .  NC_013198/1872948‑1873146                                                                                                                                                    ||                                                         gaCCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                   >  1:1058725/1‑151    cGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                <  2:534345/151‑1      ggCTTTGGTCTTTGCGCCCAAGGTCCTTAGGTGCAGTTTTCGGGGCTGGCGCACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:569761/151‑1      ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:812105/151‑1      ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:414993/151‑1      ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              >  1:133332/1‑151      ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  1:1052621/151‑1       gCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTGGGGC.GTGATGGCCCGGGCT.CGGCCATTGCGACCAAGGTCCTTacact                                             >  2:829440/1‑150        cTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacactc                                            <  2:1201137/151‑3              agaggaagcCCAAGGTCCTTATGTGCGCGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacactccgatcc                                      <  1:716518/144‑9                            gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGACCCGGCCCGCTTAGGGATCGGAGCGAAAGTGCCCTTGGGCG.TGATGGCCTGGGCT.CGGCCATTGCGACCCAGGTCCTTACACTCCGATCCCTGCGCCggtgtac                        >  2:923121/1‑146                            gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAAc                        >  2:521532/1‑151                            gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACCCCGAACCCTGCGCCGGTGAAc                        >  2:1035921/1‑151                               cttacactccgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAAc                        >  1:1254860/16‑151                                                 ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc   <  2:1099270/151‑1                                                 ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc   <  2:837939/151‑1                                                     ggtgAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGATCCg  >  2:624481/4‑151                                                                                                                                                    ||                                                         GACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCG  .  NC_013198/1872948‑1873146 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 16 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG