Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	985,365	A→G	I904I (ATT→ATC)	pacL	cation‑transporting ATPase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	985,365	0	A→G	100.0%	191.7	48	I904I (ATT→ATC)	pacL	cation‑transporting ATPase
Reads supporting (aligned to +/- strand):  new base (20/28):  ref base (0/0):  total (20/28)

AAAGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGAATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGATTGGCA  .  NC_013198/985222‑985506                                                                                                                                                |                                                                                                                                              aaaGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTCCCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGga                                                                                                                                        <  2:239223/151‑1 aaaGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGga                                                                                                                                        <  2:526124/151‑1  aaGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGgat                                                                                                                                       <  2:728214/151‑1  aaGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGgat                                                                                                                                       <  1:46764/151‑1        cacTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGc                                                                                                                                 >  1:1204844/1‑151              aaaaTGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGaa                                                                                                                           >  1:810388/1‑151              aaaaTGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGaa                                                                                                                           <  1:863731/151‑1                         ggAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCgg                                                                                                                <  1:870469/151‑1                              gTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAgg                                                                                                           >  2:867897/1‑151                                 cAAATTCCAATGCCATTTTCTTAGTGTTGCTTATTCGACGCGCTTTACCTTCTCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCt                                                                                                        <  1:1215541/151‑1                                 cAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCt                                                                                                        <  2:1204478/151‑1                                 cAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCt                                                                                                        <  1:116203/151‑1                                 cAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCt                                                                                                        <  1:796037/151‑1                                     ttCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTg                                                                                                    <  1:408629/151‑1                                     ttCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTg                                                                                                    <  2:452137/151‑1                                      tCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTgg                                                                                                   <  2:685156/151‑1                                         aaGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGcc                                                                                                <  1:1177841/151‑1                                                  ttCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGaa                                                                                       >  2:233413/1‑151                                                   gacaggtgtTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAag                                                                                      <  1:282124/146‑1                                                     ttAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAagag                                                                                    <  1:835657/151‑1                                                        gtgtTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAagctgtc                                                                                 >  2:282138/1‑146                                                             gCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTa                                                                            >  1:1025138/1‑151                                                                         cccTTTACCTTCGCATGGGCTAAGCGCCGGCGATTGAGCCATTTCCGTCCTTCTTCGTTTAGGAAGAGAAGGACCGGGATACTGGCTAGTAAGGTCCAATCGGAGAGGGCTAGCGGGTCCGTGTGGAAGAGTTTATGTTAGATCggggcgt                                                                >  2:184865/3‑148                                                                         cGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACggggggt                                                                >  2:1057498/1‑148                                                                         cGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGgtgt                                                                >  1:711052/1‑151                                                                         cGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGgtgt                                                                >  2:445784/1‑151                                                                           cTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTaa                                                              >  2:29198/1‑151                                                                            tttACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAAc                                                             <  2:359143/151‑1                                                                            tttACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAAc                                                             <  1:223489/151‑1                                                                              tACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAGGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTg                                                           <  2:972880/151‑1                                                                               aCCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGa                                                          >  2:1144141/1‑151                                                                                ccTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAg                                                         <  2:1148238/151‑1                                                                                 cTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGt                                                        <  1:1144507/151‑1                                                                                      cATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGc                                                   <  1:396184/151‑1                                                                                      cATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGc                                                   <  1:1074507/151‑1                                                                                      cATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGc                                                   <  2:777088/151‑1                                                                                      cATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGc                                                   <  1:868214/151‑1                                                                                          ggCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCcagc                                               >  1:1214941/1‑151                                                                                                 cgcCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGaaaa                                        <  1:211163/151‑1                                                                                                  gcCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAAt                                       >  1:743808/1‑151                                                                                                         aTTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAg                                >  1:1050027/1‑151                                                                                                             aGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTTAGTAGGGCCAGCAGGAAAATTTCAAAGATaa                            >  2:602668/1‑151                                                                                                                 aTTTCCGTCCTTCTTCGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGcc                        <  1:1074405/151‑1                                                                                                                                cGATTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCg         <  2:1069261/151‑1                                                                                                                                  aTTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGAt       >  2:135413/1‑151                                                                                                                                  aTTAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGAt       >  2:544079/1‑151                                                                                                                                    tAAGAAGAGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGGGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGATTg     >  2:101177/1‑151                                                                                                                                       gaagaGTGGGATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGATTGGca  >  1:854251/1‑151                                                                                                                                                |                                                                                                                                              AAAGTGACACTAAAAAATGGCCTCGGAAAGTTCAAATTCCAAGGCCATTTTCTTAGTGTTGCTTATTCGACACGCTTTACCTTCGCATGGGCTAAGCGCCGACGATTGAGCCATTTCCGTCCTTCTTCGATTAAGAAGAGTGGAATCGGGATGATGGCTAGGAAGATCCAATCGGTGAGGGCTAGTGGGCCCGTGTGGAAGAGTTCCTGTAAGAACGGGGTGTAACTGAGTAGGGCCAGCAGGAAAATTTCAAAGATAATGCCGAACCAGACAAGTCGATTGGCA  .  NC_013198/985222‑985506 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG