Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	615,483	T→C	S422P (CTG→CCG)	treB	PTS system beta‑glucoside‑specific transporter subunit IIABC

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	615,483	0	T→C	100.0%	350.5	88	S422P (CTG→CCG)	treB	PTS system beta‑glucoside‑specific transporter subunit IIABC
Reads supporting (aligned to +/- strand):  new base (41/47):  ref base (0/0):  total (41/47)

CTGCTGGCAAAGTGACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCTGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTATTCAGG  .  NC_013198/615345‑615626                                                                                                                                           |                                                                                                                                                ctgctgGCAAAGTGACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAagag                                                                                                                                     <  2:951540/151‑1       gCAAAGTGACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGAt                                                                                                                               <  1:806880/151‑1            gTGACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGc                                                                                                                          <  1:335882/151‑1              gACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCgatg                                                                                                                        >  2:837691/1‑148              gACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGGGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTg                                                                                                                        >  2:60962/1‑151                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGTTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  2:716413/151‑1                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  1:542188/151‑1                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  1:671331/151‑1                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  1:435365/151‑1                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  1:338302/151‑1                  ggCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTAc                                                                                                                    <  1:968920/151‑1                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  1:562668/1‑151                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  1:997980/1‑151                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  2:1058301/1‑151                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  2:322203/1‑151                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  1:119509/1‑151                   gCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGGGCACATTGCCAATTACACGTATTCCATTTTCCCGAGGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGGGGGTTAAGAGTTGGATGCCGCTGGTACt                                                                                                                   >  1:202503/1‑151                      caGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTgcg                                                                                                                >  2:828425/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGATTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:602093/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  1:791818/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:544499/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:579914/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:857836/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  1:647234/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:650636/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  1:284179/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  1:39890/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  1:446306/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:948242/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCa                                                                                                              >  2:981466/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGGACTGCGCa                                                                                                              >  2:208516/1‑151                        gtgtTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGCGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGGACTGCGCa                                                                                                              >  2:567668/1‑151                            tAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGAt                                                                                                          <  1:50687/151‑1                            tAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGAt                                                                                                          <  2:791628/151‑1                             aGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGAtt                                                                                                         >  2:1204662/1‑151                              ggCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGAttt                                                                                                        >  1:679415/1‑151                                  tGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTcac                                                                                                    <  2:71277/151‑1                                     gCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACAcc                                                                                                 >  1:945894/1‑151                                            cgcgAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTc                                                                                          <  1:1205028/151‑1                                            cgcgAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTc                                                                                          <  2:1079699/151‑1                                            cgcgAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTc                                                                                          <  2:208415/151‑1                                                          gCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCttggt                                                                            <  2:721162/151‑1                                                          gCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCttggt                                                                            >  2:995367/1‑151                                                               ccGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTa                                                                       >  2:26108/1‑151                                                                 ggTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATg                                                                     >  2:28088/1‑151                                                                   tGCACATTGCCAATTACGCGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGAc                                                                   <  1:1168869/151‑1                                                                    gCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACa                                                                  >  1:148342/1‑151                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGGGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  2:133132/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  2:169635/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  1:567819/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  1:580065/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  1:26108/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  1:506139/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  1:698432/151‑1                                                                     cacaTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAg                                                                 <  2:871963/151‑1                                                                       caTTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGtt                                                               >  2:508562/1‑151                                                                           gCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCt                                                           <  1:322194/151‑1                                                                             cAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTg                                                         >  2:113007/1‑151                                                                               aTTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGc                                                       >  2:936866/1‑151                                                                                ttACCCGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCg                                                      <  2:594309/151‑1                                                                                ttACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCg                                                      <  1:981780/151‑1                                                                                   cacGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGtt                                                   >  2:102755/1‑151                                                                                      gTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTggg                                                >  2:713051/1‑151                                                                                       tATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGc                                               >  2:333024/1‑151                                                                                          tCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCAc                                            <  1:1233047/151‑1                                                                                                    tgtataagagacaggCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGt                                  <  2:1109960/137‑1                                                                                                    cGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGt                                  <  1:188981/151‑1                                                                                                    cGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGt                                  <  1:346584/151‑1                                                                                                    cGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGt                                  <  2:647102/151‑1                                                                                                      atgatTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTc                                <  1:828742/151‑1                                                                                                         atTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGt                             >  1:320160/1‑151                                                                                                          tttttGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGGCACTCATCACGGTTCTGTc                            >  2:279059/1‑151                                                                                                           ttttGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCt                           <  2:1015878/151‑1                                                                                                           ttttGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCt                           <  1:948556/151‑1                                                                                                           ttttGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCt                           <  1:753111/151‑1                                                                                                           ttttGCAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCt                           <  1:308344/151‑1                                                                                                                cAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATgg                      <  1:713196/151‑1                                                                                                                cAGCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATgg                      <  1:650768/151‑1                                                                                                                  gCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCa                    >  2:781697/1‑151                                                                                                                  gCGTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGT.CTGTCtcttataca                   >  1:1110328/1‑143                                                                                                                    gTGGATGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATc                  >  1:137690/1‑151                                                                                                                        aTGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAg              <  2:945580/151‑1                                                                                                                         tGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGc             <  2:562517/151‑1                                                                                                                         tGGCGGCAAAAATCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGc             <  1:460817/151‑1                                                                                                                                aaaaaTCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTAtt      <  1:781884/151‑1                                                                                                                                 aaaaTCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTATTc     <  2:446317/151‑1                                                                                                                                 aaaaTCGAGCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTATTc     <  2:320169/151‑1                                                                                                                                    aTCGATCCGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTATTCAgg  <  1:775788/151‑1                                                                                                                                           |                                                                                                                                                CTGCTGGCAAAGTGACCGGCACAGTGTTAGGCATGGGCATCAACGCGAACTTCTTCGGCTTGCCGGTGCACATTGCCAATTACACGTATTCCATTTTCCCGATGATTTTTGCAGCGTGGATGGCGGCAAAAATCGAGCTGTGGATTAAGAGTTGGATGCCGCTGGTACTGCGCATGATTTTCACACCATTAGTCGAAATCTTCTTGGTTGGTATGACAGTTGTCTTGGCGGTTGGGCCACTGATCACGGTTCTGTCTAATGGCATCACAGCTGGTATTCAGG  .  NC_013198/615345‑615626 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG