Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	2,160,010	G→A	A459T (GCG→ACG)	LGG_02095	type II restriction enzyme methylase subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	2,160,010	0	G→A	100.0%	326.3	93	A459T (GCG→ACG)	LGG_02095	type II restriction enzyme methylase subunit
Reads supporting (aligned to +/- strand):  new base (20/65):  ref base (3/5):  total (23/70)

GCCTTTATTATTCAAAAGCAAGCGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCTTTCTACGTCAA  .  NC_013198/2159863‑2160159                                                                                                                                                    |                                                                                                                                                      gCCTTTATTATTCAAAAGCAAGCGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGa                                                                                                                                                    >  2:820178/1‑151          attCAAAAGCAAGCGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTgaga                                                                                                                                           >  2:358695/1‑151          attCAAAAGCAAGCGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTgaga                                                                                                                                           <  2:801579/151‑1                 agcaagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATAcc                                                                                                                                    <  1:935162/151‑1                 agcaagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATAcc                                                                                                                                    <  1:577315/151‑1                 agcaagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATAcc                                                                                                                                    <  2:544015/151‑1                  gcaagcGAGTTCAAACTTTAGCGGCACTTATGAGCGTCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATACCt                                                                                                                                   <  1:100291/151‑1                   caagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTg                                                                                                                                  <  1:603608/151‑1                    aagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTgg                                                                                                                                 <  2:83781/151‑1                    aagcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTgg                                                                                                                                 <  2:8093/151‑1                     agcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGa                                                                                                                                <  1:8973/151‑1                     agcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGa                                                                                                                                <  2:117286/151‑1                     agcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGa                                                                                                                                >  2:674904/1‑151                     agcGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGa                                                                                                                                <  1:265766/151‑1                         aGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGCTTTTGATTCACAAGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAATGAACTTTGAGAAGATACCTGGAAGTc                                                                                                                            >  2:340824/1‑151                          gTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTcc                                                                                                                           >  1:678695/1‑151                          gTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGATTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACATTGAGAAGAAACCTGGAAGTcc                                                                                                                           >  2:871097/1‑151                             cAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGAt                                                                                                                        <  1:198125/151‑1                             cAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGAt                                                                                                                        <  1:381528/151‑1                               aaCTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAg                                                                                                                      <  2:60481/151‑1                               aaCTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAg                                                                                                                      <  1:384657/151‑1                               aaCTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAg                                                                                                                      <  1:1077047/151‑1                                aCTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGc                                                                                                                     <  2:1056287/151‑1                                 cTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCt                                                                                                                    >  1:74378/1‑151                                        ggCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTggg                                                                                                             <  1:725252/151‑1                                        ggCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTggg                                                                                                             <  1:57660/151‑1                                        ggCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTggg                                                                                                             <  2:324411/151‑1                                        ggCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTggg                                                                                                             <  1:1143146/151‑1                                        ggCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTggg                                                                                                             <  1:364343/151‑1                                         gCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGc                                                                                                            >  2:1036204/1‑151                                         gCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGc                                                                                                            >  1:29158/1‑151                                            cTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAg                                                                                                         >  2:915817/1‑151                                            cTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAg                                                                                                         >  1:929795/1‑151                                            cTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAg                                                                                                         >  1:1032350/1‑151                                                   gCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  1:577317/151‑1                                                   gCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  1:1084702/151‑1                                                   gCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  2:184612/151‑1                                                   gCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  1:483172/151‑1                                                   gCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  1:678129/151‑1                                                   gCGGATGGTCGATTTTGATTCACAGGCCCATAAGGAAAACTCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTCCTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAAccc                                                                                                  <  2:1023763/151‑1                                                    cGGCTGGTCGATTTTGATTCACAGGCTCATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATTCCTGGAAGTCCGATAGCTTATTGGGCGAGTaatcctg                                                                                                 <  2:98045/151‑6                                                         ggTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTAtt                                                                                            <  2:340099/151‑1                                                           tCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTc                                                                                          <  2:30463/151‑1                                                           tCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTc                                                                                          <  2:895558/151‑1                                                                             cacaTAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCAt                                                                        >  1:37708/1‑151                                                                               caTAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATAc                                                                      >  1:917667/1‑151                                                                                aTAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATAcc                                                                     <  1:636788/151‑1                                                                                aTAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATAcc                                                                     <  1:757795/151‑1                                                                                aTAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATAcc                                                                     <  1:390070/151‑1                                                                                 tAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCa                                                                    <  2:605564/151‑1                                                                                  aaGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCAc                                                                   <  2:417354/151‑1                                                                                    ggAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTc                                                                 >  1:765325/1‑151                                                                                       aaaaGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCaaa                                                              <  2:868624/151‑1                                                                                         aaGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGa                                                            >  1:726750/1‑151                                                                                          aGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGAc                                                           >  2:943898/1‑151                                                                                          aGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGAc                                                           <  2:159945/151‑1                                                                                          aGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGAc                                                           <  2:962190/151‑1                                                                                          aGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGAc                                                           <  2:325309/151‑1                                                                                            cTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACtt                                                         <  1:953939/151‑1                                                                                               aTCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGt                                                      >  2:395165/1‑151                                                                                                tCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATGCCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGtt                                                     <  2:102786/151‑1                                                                                                tCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGtt                                                     <  1:1114343/151‑1                                                                                                tCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGtt                                                     <  2:457094/151‑1                                                                                                acTTGCTGCCGTTAAAGAACCAAATCTTAAGTATGTTTATCGTACTAAACGAACGAACTTTGAGAAGATACCTGGAAGTTCGATAGCTTATTGGGCGAGTAAACCCTTTATTTCCGATTTTGAAATTGGCATACCACGCAAAGACTTAgat                                                     <  2:788114/150‑3                                                                                                 cTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTg                                                    <  1:236648/151‑1                                                                                                 cTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTg                                                    <  1:1078980/151‑1                                                                                                 cTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTg                                                    <  1:557163/151‑1                                                                                                 cTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTg                                                    <  2:1057674/151‑1                                                                                                       gcCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTa                                              >  1:1237506/1‑151                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  1:248209/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  2:765143/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  1:599192/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  1:34512/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  2:37708/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  1:982048/151‑1                                                                                                          gTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAg                                           <  1:764674/151‑1                                                                                                           ttAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGt                                          <  2:252153/151‑1                                                                                                              aaGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTggg                                       <  2:1245745/151‑1                                                                                                                gATCCAAATCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTc                                     <  1:1053386/151‑1                                                                                                                       aTCTTAAGTATGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAAc                              >  1:467968/1‑151                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  2:793004/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  2:683502/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  1:341212/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  1:633444/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  1:1084763/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  2:917351/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  2:321476/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  1:469678/151‑1                                                                                                                                 tGTTTATCGTACTAAACAAACGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAATTGATCCTAAAGTGGGTCTGCAAACTGGTGATAAc                    <  1:484718/151‑1                                                                                                                                         gTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCATAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCtt            >  2:836722/1‑151                                                                                                                                         gTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCtt            >  2:348498/1‑151                                                                                                                                               aaCAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCTTTCTACg      <  2:743909/151‑1                                                                                                                                                   aGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCTTTCTACGTCaa  >  2:895018/1‑151                                                                                                                                                    |                                                                                                                                                      GCCTTTATTATTCAAAAGCAAGCGAGTTCAAACTTTAGCGGCACTTATGAGCGGCTGGTCGATTTTGATTCACAGGCACATAAGGAAAAAGCTTATCTTGCTGCCGTTAAAGATCCAAATCTTAAGTATGTTTATCGTACTAAACAAGCGAACTTTGAGAAGATACCTGGAAGTCCGATAGCTTATTGGGCGAGTAAACCCCTTATTTCCGATTTTGAAATTGGCATACCACTCAAAGACTTAGTTGATCCTAAAGTGGGTCTGCAAACTGGTGATAACAGTCGCTTTCTACGTCAA  .  NC_013198/2159863‑2160159 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG