Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,122	C→T	R228H (CGC→CAC)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,122	0	C→T	100.0%	44.4	16	R228H (CGC→CAC)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/7):  ref base (2/0):  total (9/7)

GTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCG  .  NC_013198/1872975‑1873266                                                                                                                                                        |                                                                                                                                                   gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAAc                                                                                                                                                >  2:521532/1‑151 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACCCCGAACCCTGCGCCGGTGAAc                                                                                                                                                >  2:1035921/1‑151    cttacactccgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAAc                                                                                                                                                >  1:1254860/16‑151                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc                                                                                                                           <  2:1099270/151‑1                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc                                                                                                                           <  2:837939/151‑1                          ggtgAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGATCCg                                                                                                                          >  2:624481/4‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg                         >  2:1253928/1‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg                         >  2:381623/1‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg                         >  2:542582/1‑151                                                                                                                                     actccgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGtt                 >  1:1112915/11‑151                                                                                                                                         cgatcccTGGGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttatgg             <  2:1077353/145‑5                                                                                                                                         cgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttatgg             <  1:1254294/145‑5                                                                                                                                         cgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttatgg             <  1:521683/145‑5                                                                                                                                           atcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttatggtg           <  1:987804/147‑7                                                                                                                                                    ccGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttctggggagcgtgatc  >  2:507745/1‑136                                                                                                                                                    ccGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGttatggtgagcgtgatc  <  1:627165/151‑16                                                                                                                                                        |                                                                                                                                                   GTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCG  .  NC_013198/1872975‑1873266 Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG