Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,105	G→T	R234S (CGT→AGT)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,105	0	G→T	100.0%	18.8	10	R234S (CGT→AGT)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (2/0):  total (8/2)

GTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCG  .  NC_013198/1872975‑1873243                                                                                                                                       |                                                                                                                                             gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGACCCGGCCCGCTTAGGGATCGGAGCGAAAGTGCCCTTGGGCG.TGATGGCCTGGGCT.CGGCCATTGCGACCCAGGTCCTTACACTCCGATCCCTGCGCCggtgtac                                                                                                                         >  2:923121/1‑146 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAAc                                                                                                                         >  2:521532/1‑151 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACCCCGAACCCTGCGCCGGTGAAc                                                                                                                         >  2:1035921/1‑151    cttacactccgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAAc                                                                                                                         >  1:1254860/16‑151                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc                                                                                                    <  2:1099270/151‑1                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc                                                                                                    <  2:837939/151‑1                          ggtgAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGATCCg                                                                                                   >  2:624481/4‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg  >  2:1253928/1‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg  >  2:381623/1‑151                                                                                                                             gTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCg  >  2:542582/1‑151                                                                                                                                       |                                                                                                                                             GTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCG  .  NC_013198/1872975‑1873243 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 17 ≤  ATCG  < 30 ≤  ATCG  < 39 ≤  ATCG