Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,058	A→G	P249P (CCT→CCC)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,058	0	A→G	100.0%	100.1	26	P249P (CCT→CCC)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/15):  ref base (0/0):  total (11/15)

GGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCG  .  NC_013198/1872911‑1873146                                                                                                                                                       |                                                                                            ggTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGc                                                                                          <  1:406449/151‑1 ggTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGc                                                                                          <  1:789753/151‑1               gCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcctgggc                                                                           <  1:382662/151‑6                  tAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCttg                                                                        >  2:361175/1‑150                     gTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCttggcc                                                                     <  1:361166/151‑5                     gTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCttggcc                                                                     <  1:806762/151‑5                     gTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCttggcc                                                                     <  2:670468/151‑5                        gCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCAtt                                                                 >  1:493764/1‑151                         ccTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTg                                                                >  2:488226/1‑151                                      gaCCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                   >  1:1058725/1‑151                                         cGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                <  2:534345/151‑1                                           ggCTTTGGTCTTTGCGCCCAAGGTCCTTAGGTGCAGTTTTCGGGGCTGGCGCACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:569761/151‑1                                           ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:414993/151‑1                                           ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  2:812105/151‑1                                           ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              >  1:133332/1‑151                                           ggCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                              <  1:1052621/151‑1                                            gCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTGGGGC.GTGATGGCCCGGGCT.CGGCCATTGCGACCAAGGTCCTTacact                                             >  2:829440/1‑150                                             cTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacactc                                            <  2:1201137/151‑3                                                   agaggaagcCCAAGGTCCTTATGTGCGCGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacactccgatcc                                      <  1:716518/144‑9                                                                 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGACCCGGCCCGCTTAGGGATCGGAGCGAAAGTGCCCTTGGGCG.TGATGGCCTGGGCT.CGGCCATTGCGACCCAGGTCCTTACACTCCGATCCCTGCGCCggtgtac                        >  2:923121/1‑146                                                                 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAAc                        >  2:521532/1‑151                                                                 gTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACCCCGAACCCTGCGCCGGTGAAc                        >  2:1035921/1‑151                                                                    cttacactccgatcccTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAAc                        >  1:1254860/16‑151                                                                                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc   <  2:1099270/151‑1                                                                                      ggCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAAcc   <  2:837939/151‑1                                                                                          ggtgAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGATCCg  >  2:624481/4‑151                                                                                                                                                       |                                                                                            GGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCG  .  NC_013198/1872911‑1873146 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 20 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG