Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,561,525	C→G	intergenic (‑1158/+1687)	LGG_01544/rimL	hypothetical protein/GNAT family acetyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,561,525	0	C→G	100.0%	16.7	51	intergenic (‑1158/+1687)	LGG_01544/rimL	hypothetical protein/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  new base (0/27):  ref base (8/14):  total (9/42)

CAATCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTCTGAATACTTAGCAAG  .  NC_013198/1561376‑1561670                                                                                                                                                      |                                                                                                                                                  cAATCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCg                                                                                                                                                  >  2:440463/1‑151  aaTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCgg                                                                                                                                                 >  1:522047/1‑151   agcAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:1221801/149‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAATTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:926826/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:680069/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:973644/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:98685/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:1043223/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:1062690/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:1234921/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:238572/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:241626/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:300939/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:363696/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:413251/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:441092/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:508797/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:807874/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:995073/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:675741/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:294499/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:434132/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:662309/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:573558/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:575374/151‑1   aTCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTAAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  1:450178/151‑1   aTCAGTGGCCATCAGGCTTCGTCTTCGTTCCAAAAGAATTGAAAAGTTTAACAAAAGTTGGCATGGACGGCTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGggg                                                                                                                                                <  2:1090129/151‑1   aTCAGTGACAGTCTGGCTTCGCCTGGGTTCCACACGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGCCGGTTTTTGTCAGGTTGTGATGTCAAGGTATCCAGCAACCATGCTGGTGGGGGGAGACACCGGTTTTGGGTGTGggg                                                                                                                                                <  2:801470/151‑1    tCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTGgggc                                                                                                                                               <  2:164887/151‑2                     tCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATAc                                                                                                                              <  1:278786/151‑1                     tCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATAc                                                                                                                              <  1:343177/151‑1                          ttCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAAc                                                                                                                         <  1:593612/151‑1                          ttCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAAc                                                                                                                         <  2:209052/151‑1                                                gTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGGGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAg                                                                                                   >  2:102181/1‑151                                                               aCATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAg                                                                                    <  2:11433/151‑1                                                                       gacTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACtt                                                                            <  2:521896/151‑1                                                                            aCGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTc                                                                       <  2:425083/151‑1                                                                               gTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCAt                                                                    >  1:645172/1‑151                                                                                               tgtataagagacagcAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGc                                                    <  2:332144/137‑1                                                                                                     aaGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGc                                              <  1:17647/151‑1                                                                                                      aGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCt                                             <  1:440452/151‑1                                                                                                        gTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTc                                           >  2:1058056/1‑151                                                                                                             cAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGcctgtctcttataca                                      >  1:332135/1‑137                                                                                                                       agcttacgagggAAACACCGGTTTTTGGTGT..GGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAAcc                          <  1:581781/142‑1                                                                                                                         tGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAAcc                          <  1:852194/151‑1                                                                                                                         tGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAAcc                          <  2:184479/151‑1                                                                                                                                gggAAACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCAt                   >  1:279883/1‑151                                                                                                                                gggAAACACCGGTTTTTGGTGT..GGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTc                 >  1:1112874/1‑151                                                                                                                                         cGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATTCTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTCTGAATAc          <  1:1164582/151‑1                                                                                                                                         cGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTCTGAATAc          <  2:395419/151‑1                                                                                                                                                 ggTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTCTGAATACTTAGcgca  >  1:77663/1‑148                                                                                                                                                      |                                                                                                                                                  CAATCAGTGTCACTCTGGCTTCGCCTTCGTTCCACAAGAATTGAAAAGTTTAACAAAAGTTGACATGGACGACTGACGGTTTTTGTCAGTTGGTTATGTCAAGGTATCCAGCAACCATGCTGGCGAGGGGAGACACCGGTTTTTGGTGTCGGGGGCGAGTAGCCCCAATACTTAACTGGTAACTTAATAGTAAACTAGCTGATAACTTAATAGCATTACTTAACTCCATTCAGCCATCGCTACGCTCTAGCTTCATTAACAGTAAGCAACCTTTGCATTCTGAATACTTAGCAAG  .  NC_013198/1561376‑1561670 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG