Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,994,717	T→A	D903V (GAT→GTT)	LGG_01953	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,994,717	0	T→A	100.0%	248.4	59	D903V (GAT→GTT)	LGG_01953	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/26):  ref base (0/0):  total (33/26)

GTATTGCCGCCTTCGTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAATCATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGACG  .  NC_013198/1994573‑1994864                                                                                                                                                 |                                                                                                                                                    gTATTGCCGCCTTCGTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATAcc                                                                                                                                               <  2:622275/151‑1   aTTGCCGCCTTCGTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCa                                                                                                                                             >  1:494037/1‑151   aTTGCCGCCTTCGTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGGAGCTGATCCTCCACCAATGTAAACATAcccc                                                                                                                                             >  1:789002/1‑150               gTCTGCGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGtt                                                                                                                                 <  1:905428/151‑1               gTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGtt                                                                                                                                 <  2:925745/151‑1                    cGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTc                                                                                                                            <  2:376268/151‑1                         aCTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGc                                                                                                                       <  2:233856/151‑1                         aCTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGc                                                                                                                       <  1:556776/151‑1                                ttGTGCCGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGatc                                                                                                                <  2:648537/151‑1                                   tgACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGatcatc                                                                                                             <  1:551309/151‑1                                      cGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCTCCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAAc                                                                                                          <  2:655600/151‑1                                      cGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAAc                                                                                                          <  1:636946/151‑1                                       tgTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACg                                                                                                         <  2:409294/150‑1                                        gTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCCTCACTAGTCGTTAACCAACTTGTAGCTGATCCCCCCCCAATGTAAACATACCCATCAGTAGAAGTTAGTTCGTGGCCTTGATCATCAACGt                                                                                                        >  2:95994/1‑151                                        gTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAAGTCACTGTTGTTGTCTTTTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCACTAGAAGATAGCTCGTGGCCTTGATCATCaaact                                                                                                        >  2:986740/1‑148                                                           gATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTgatgat                                                                                     <  2:125012/151‑1                                                                gTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGttt                                                                                <  2:367516/151‑1                                                                gTGCTTGTGTAGCTTACTGTTGTTGGCTTGTGACCATCCCTAGTCGTTAACCAACTTGTTGCTGATCCTCCGCCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGttt                                                                                <  2:57226/151‑1                                                                 tGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCTTCCACCAATTTAAACATACGCATCAGTAGAAGTTAGCTCCGGGCCTTGATCTTACACGTCAAGAATCTTATTAATGATTGtttg                                                                               >  2:113848/1‑150                                                                 tGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTCATTGATGATTGTTTc                                                                               >  2:973817/1‑151                                                                     tgtgTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATTTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGttt                                                                           <  1:496175/151‑1                                                                     tgtgTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGttt                                                                           >  2:611850/1‑151                                                                      gtgtAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTg                                                                          >  2:899135/1‑151                                                                      gtgtAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTg                                                                          >  1:958092/1‑151                                                                      gtgtAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTg                                                                          >  1:363241/1‑151                                                                           ggTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCg                                                                     >  2:936161/1‑151                                                                           ggTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCg                                                                     >  2:492517/1‑151                                                                             tCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGt                                                                   >  1:40926/1‑151                                                                                cTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTAtt                                                                >  2:414364/1‑151                                                                                 tgttgttgtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTc                                                               <  1:916104/151‑1                                                                                 tgttgttgtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTc                                                               <  2:223616/151‑1                                                                                  gttgttgtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCt                                                              >  1:305295/1‑151                                                                                   ttgttgtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCtt                                                             >  1:577491/1‑151                                                                                       tgtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTc                                                         <  1:157009/151‑1                                                                                        gtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTcc                                                        >  2:507460/1‑151                                                                                        gtCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTcc                                                        >  1:626711/1‑151                                                                                                aCCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTACAAACGTTg                                                >  1:493254/1‑151                                                                                                   aTCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTc                                             <  1:772978/151‑1                                                                                                    tCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCg                                            >  2:168846/1‑151                                                                                                    tCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCg                                            >  2:150576/1‑151                                                                                                    tCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATCATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCg                                            <  2:665849/151‑1                                                                                                                 aCCAACTTGCAGCTGATCCTCCACCAATGTAAACATACCCATCCGAAGAAGTTAGCTCGTCGCCTTGCACATCAACGTTCACAAACTTATTGATGATTTTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGCTCTTGGTGAAGgttgt                               >  2:563095/1‑151                                                                                                                   cAACTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGttgttg                             <  1:931799/151‑1                                                                                                                     aCTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTc                           >  1:88629/1‑151                                                                                                                     aCTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTc                           >  1:66143/1‑151                                                                                                                     aCTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTc                           >  1:800237/1‑151                                                                                                                     aCTTGTAGCTGATCCTCCACAAATGTAAACATACCCATCAGTAGAAGTTAGCTAGTGGCCTTGAACATCAACGTTAACAAACTTATTGATGATTTTTTCGTTTGGTTCGGTATTCTTTTTCCAAACTTTGGTCGTGGTGACGGTTGTTGTc                           >  1:934601/1‑151                                                                                                                      cTTGTAGCTGATCCTCCACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCg                          >  1:797471/1‑151                                                                                                                                 tcctccACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTggg               >  1:856279/1‑151                                                                                                                                  cctccACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGt              <  2:509323/151‑1                                                                                                                                   ctccACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGtc             <  2:761242/151‑1                                                                                                                                   ctccACCAATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGtc             <  1:701212/151‑1                                                                                                                                     ccaccaATGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGtctt           >  1:897558/1‑151                                                                                                                                          aaTGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTa      >  2:774612/1‑151                                                                                                                                          aaTGTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTa      >  2:601850/1‑151                                                                                                                                             gTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGAc   >  1:343524/1‑151                                                                                                                                             gTAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGAc   >  1:262338/1‑151                                                                                                                                              tAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGACg  <  1:27172/151‑1                                                                                                                                              tAAACATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGACg  <  1:168838/151‑1                                                                                                                                                 |                                                                                                                                                    GTATTGCCGCCTTCGTCTACGTCAACTTCTTTTGTGACGGTTTTGGCTTGTGGCTTGTGATACGTGCTTGTGTAGGTCACTGTTGTTGTCTTGTGACCATCACTAGTCGTTAACCAACTTGTAGCTGATCCTCCACCAATGTAATCATACCCATCAGTAGAAGTTAGCTCGTGGCCTTGATCATCAACGTTAACAAACTTATTGATGATTGTTTCGTTTGGATCGGTATTCTTTTTCCAAACGTTGGTCGTGGTGAAGGTTGTTGTCGATGAGCTTGGGTCTTCTTTAGACG  .  NC_013198/1994573‑1994864 Base quality scores:  ATCG  < 3 ≤  ATCG  < 9 ≤  ATCG  < 25 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG