Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,372,183	T→A	L406M (TTG→ATG)	ycjM	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,372,183	0	T→A	100.0%	245.7	59	L406M (TTG→ATG)	ycjM	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (26/33):  ref base (0/0):  total (26/33)

TAATCACGATGCGCGATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAATTGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTCA  .  NC_013198/1372049‑1372327                                                                                                                                       |                                                                                                                                                 taatCACGATGCGCGATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCcgcg                                                                                                                                  >  2:394246/1‑151           gcgcGATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACgg                                                                                                                        >  2:121358/1‑151             gcgATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGcc                                                                                                                      >  2:348275/1‑151             gcgATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGcc                                                                                                                      >  2:694660/1‑151                  gttgtCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAAc                                                                                                                 <  1:716546/151‑1                   ttgtCACTAATTATATTTATCCCAACCCTTTGTCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAAcc                                                                                                                <  1:754132/151‑1                   ttgtCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAAcc                                                                                                                <  1:394266/151‑1                   ttgtCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAAcc                                                                                                                <  2:75634/151‑1                   ttgtCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAAcc                                                                                                                <  2:2544/151‑1                        aCTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCgcagc                                                                                                           <  2:493158/151‑1                         cTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCgcagca                                                                                                          >  1:377684/1‑151                               atatTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACaa                                                                                                    <  1:21400/151‑1                                  tttATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTa                                                                                                 <  2:437692/151‑1                                     aTCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGa                                                                                              <  1:337763/151‑1                                      tCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGat                                                                                             >  2:802142/1‑151                                       cccAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGata                                                                                            <  1:877699/151‑1                                            cacTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTAc                                                                                       >  2:430343/1‑151                                            cacTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTAc                                                                                       >  2:462991/1‑151                                            cacTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAAAGGCCTAAACCGCAGCACTACAATTACGATATGTAc                                                                                       >  2:982151/1‑151                                             ccTTTGTCGGTGCTGCGGCTGACGGGTGCTGAACTGAAGGCGGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGCCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTAcc                                                                                      <  2:764002/150‑1                                             ccTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTAcc                                                                                      <  1:694893/150‑1                                              cTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCa                                                                                     >  1:675596/1‑151                                               tttGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCaa                                                                                    <  1:218942/151‑1                                                 tGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAgg                                                                                  <  1:639267/151‑1                                                 tGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAgg                                                                                  <  2:642120/151‑1                                                 tGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAgg                                                                                  <  2:380990/151‑1                                                 tGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAgg                                                                                  <  1:922676/151‑1                                                 tGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTAATTATTTTCCCCTAAATGCTCAGGGTAAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAgg                                                                                  <  1:2457/151‑1                                                 ggggtgtgccgggtcTGAGTGGTGCAGAACTGAGGGCGGCGTTAGAGCGGTGTGCGACTTATTGTTCCGTAAAGGCTCGGGGACGAATGATGAGGGCGCCGCGTTTTGAACGGCGTAAACGGCGGCACTACAATTACGATATGTACCAAgg                                                                                  <  2:817814/137‑1                                                  ggCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGc                                                                                 <  2:716867/151‑1                                                  ggCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGc                                                                                 <  1:150146/151‑1                                                             ggCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATAc                                                                      <  2:898197/151‑1                                                             ggCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATAc                                                                      <  1:455647/151‑1                                                                      gTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGatat                                                             >  1:283972/1‑151                                                                         ctgaactgaaGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTc                                                          <  2:442749/151‑1                                                                           gaactgaaGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCa                                                        >  1:787357/1‑151                                                                           gaactgaaGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCa                                                        >  1:480225/1‑151                                                                                       gcgTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAAc                                            <  2:904396/151‑1                                                                                       agagacaggCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAAc                                            <  1:168313/143‑1                                                                                        cgTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACa                                           >  2:275749/1‑151                                                                                        cgTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACa                                           >  2:742457/1‑151                                                                                        cgTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACa                                           >  1:351344/1‑151                                                                                        cgTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCATAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACa                                           >  1:858762/1‑151                                                                                         gTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAg                                          <  2:620126/151‑1                                                                                         gTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAg                                          <  2:548671/151‑1                                                                                         gTTAGAGCAGTGGGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAg                                          <  2:860993/151‑1                                                                                          ttAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAgc                                         >  1:152739/1‑151                                                                                          ttAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAgc                                         >  2:214959/1‑151                                                                                               gCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAAcctgtctct                                    >  2:168321/1‑143                                                                                                                tttCCCTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCa                   <  1:214951/151‑1                                                                                                                    ccTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGc               <  1:559245/151‑1                                                                                                                    ccTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGc               >  1:664787/1‑151                                                                                                                     cTAAATGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCa              >  2:491853/1‑151                                                                                                                         aTGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGcc          <  2:283971/151‑1                                                                                                                          tGCTCAGGGTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGccc         >  1:181116/1‑151                                                                                                                                gggTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTc   <  2:599935/151‑1                                                                                                                                gggTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTc   >  2:53192/1‑151                                                                                                                                gggTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTc   >  1:275839/1‑151                                                                                                                                 ggTCAAATGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTCa  >  2:436743/1‑151                                                                                                                                       |                                                                                                                                                 TAATCACGATGCGCGATGTTGTCACTAATTATATTTATCCCAACACTTTGGCGGTGCTGCGGCTGACTGGTGCTGAACTGAAGGCCGCGTTAGAGCAGTGTGCTACTTATTTTTCCCTAAATGCTCAGGGTCAATTGATGGTCACGCCGCGATTTGAACGGCCTAAACCGCAGCACTACAATTACGATATGTACCAAGGCATTGATTATACTTTGGATATTTCCAAACCGGTTAAACAGCGCGTCACGAAACTAACTTATCATGGCAAGCCCGTGGTCA  .  NC_013198/1372049‑1372327 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG