Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	2,237,063	0	T→G	100.0%	1.8	2	L35W (TTG→TGG)	is60	transposase IS5 family protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/0):  total (0/2)

TTTGAATCCTTTGGAAGCGCTCTGGGAACACCGCTCAGCCCGGACAACGAGTGGGTTCAATATGCAGAATTGATCCCATGGGCCAAACTTGAAGAGGCATAC  >  NC_013198/2236993‑2237094                                                                       |                                tttGA.TCCTTTGGAACCGCTGCGGGAACTCCTATTATGCCGGTACACGAGATGGTTCAATATGCAGAATTTATCCCATGGACCAAACTTGAAGAGTCATAc  <  13:912416/101‑1 tttGA.TCCTTTGGAACCGCTGCGGGAACTCCTATTATGCCGGTACACGAGATGGTTCAATATGCAGAATTTATCCCATGGACCAAACTTGAAGAGTCATAc  <  29:912416/101‑1 tttGA.TCCTTGTGAAGCGCTCTGGGGACACCGCTCAGCCCGGACAACGAGTGGGGTCAATATGCAGAATGGATCCCATGGGCCAAACTTGAAGAGGCATAc  <  18:724012/101‑1 tttGA.TCCTTGTGAAGCGCTCTGGGGACACCGCTCAGCCCGGACAACGAGTGGGGTCAATATGCAGAATGGATCCCATGGGCCAAACTTGAAGAGGCATAc  <  2:724012/101‑1                                                                       |                                TTTGAATCCTTTGGAAGCGCTCTGGGAACACCGCTCAGCCCGGACAACGAGTGGGTTCAATATGCAGAATTGATCCCATGGGCCAAACTTGAAGAGGCATAC  >  NC_013198/2236993‑2237094 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 10 ≤  ATCG  < 17 ≤  ATCG  < 35 ≤  ATCG