Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,071	+T	coding (734/795 nt)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,071	1	.→T	100.0%	105.4	38	coding (734/795 nt)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/22):  ref base (6/8):  total (8/30)

CCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACG  >  NC_013198/1872970‑1873127                                                                                                          |                                                         ccAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcc                                                           >  12:1227605/1‑101 ccAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcc                                                           >  29:1065974/1‑101 ccAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcc                                                           >  28:1227605/1‑101 ccAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcc                                                           >  13:1065974/1‑101  cAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGGGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcct                                                          <  8:592844/101‑2  cAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGGGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGcct                                                          <  24:592844/101‑2       tCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAGCCGGCGCGCTTAGAAACCGGAGTGTAAGTGGCCTTGGGCG.TAATGGCC.GGGCtt                                                    <  4:1169113/101‑1       tCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAGCCGGCGCGCTTAGAAACCGGAGTGTAAGTGGCCTTGGGCG.TAATGGCC.GGGCtt                                                    <  20:1169113/101‑1        ccTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGc                                                      >  9:51375/1‑101        ccTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGc                                                      >  25:51375/1‑101        ccTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCtt                                                    <  4:242851/101‑1        ccTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCtt                                                    <  20:242851/101‑1        acTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCtt                                                    >  25:875897/2‑101        acTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCtt                                                    >  9:875897/2‑101           tATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCttt                                                   <  12:1181212/101‑1           tATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCttt                                                   <  28:1181212/101‑1            aTGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  <  6:521397/101‑1            aTGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  <  11:109957/101‑1            aTGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  <  22:521397/101‑1            aTGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  <  27:109957/101‑1            aTGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  >  30:1181165/1‑101            aTGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCG.TGATGACC.GGGCTTTg                                                  >  14:1181165/1‑101              gtgCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCAt                                             <  26:336055/101‑1              gtgCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCAt                                             <  10:336055/101‑1                gCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTg                                           >  21:977866/1‑101                gCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTg                                           >  5:977866/1‑101                 cAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGc                                          <  7:1050850/101‑1                 cAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGc                                          <  23:1050850/101‑1                            gCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                               <  15:973349/101‑1                            gCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                               <  31:973349/101‑1                            gCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGACCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                               <  26:500727/101‑1                            gCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGACCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                               <  10:500727/101‑1                              tGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                             <  5:25333/101‑1                              tGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                             <  21:25333/101‑1                               ggCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                            <  11:413806/101‑1                               ggCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                            <  27:413806/101‑1                               ggCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                            <  28:703468/101‑1                               ggCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                            <  12:703468/101‑1                                gCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                           <  1:667962/101‑1                                gCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                           <  17:667962/101‑1                                   aaCGCGTTATG...AGCGTGAACCTTCGCGCTTATTGATCGGAGGGTAAGGGGCCT.ATGCCGTGAGCGCC.TGTGCTTGGCCATCGCGACTAAGGTCCTCacacc                        <  28:943403/101‑2                                   aaCGCGTTATG...AGCGTGAACCTTCGCGCTTATTGATCGGAGGGTAAGGGGCCT.ATGCCGTGAGCGCC.TGTGCTTGGCCATCGCGACTAAGGTCCTCacacc                        <  12:943403/101‑2                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGGTCGGAGTGTAAGTGGGCTTGGGCG.TGATGGCCTGGGCTT.GGCCCTTGCGACCAAGGGCCTTacact                        >  6:149577/1‑100                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGGTCGGAGTGTAAGTGGGCTTGGGCG.TGATGGCCTGGGCTT.GGCCCTTGCGACCAAGGGCCTTacact                        >  22:149577/1‑100                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                        <  12:498787/101‑2                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                        <  28:498787/101‑2                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                        <  24:1057494/101‑2                                   aaCGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                        <  8:1057494/101‑2                                                            gcgcggTTAGCAATCGGAGTGTAAGTGGCCTTAAGCG.TGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAAcg  >  7:385848/1‑101                                                            gcgcggTTAGCAATCGGAGTGTAAGTGGCCTTAAGCG.TGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAAcg  >  23:385848/1‑101                                                                                                          |                                                         CCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATG...AGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACG  >  NC_013198/1872970‑1873127 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG