Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,091	2 bp→CA	coding (713‑714/795 nt)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,091	0	T→C	100.0%	198.5	46	S238S (TCA→TCG)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/30):  ref base (0/0):  total (16/30)
*	NC_013198	1,873,092	0	G→A	100.0%	160.3	46	S238L (TCA→TTA)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/30):  ref base (0/0):  total (16/30)

GCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGAT.GGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGAT  >  NC_013198/1872997‑1873187                                                                                                 ||                                                                                                  gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                                             >  3:1167374/1‑101 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                                             >  11:693900/1‑101 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                                             >  25:693900/1‑101 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                                             >  17:1167374/1‑101  cTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                                                            <  11:1177497/101‑1  cTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                                                            <  25:1177497/101‑1  cTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                                                            <  6:590380/101‑1  cTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCt                                                                                            <  20:590380/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           >  15:1179922/1‑101   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           >  19:948497/1‑101   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           <  26:622383/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           >  5:948497/1‑101   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           <  26:540362/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           >  1:1179922/1‑101   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           <  12:622383/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                                           <  12:540362/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          <  21:421158/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          >  18:725169/1‑101    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          <  24:882873/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          >  27:411206/1‑101    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          >  4:725169/1‑101    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          <  7:421158/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          >  13:411206/1‑101    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                                          <  10:882873/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  10:104381/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  6:440190/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  7:863562/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  21:863562/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  20:440190/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                                         <  24:104381/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTTGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       >  18:1023434/1‑101       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTTGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       >  4:1023434/1‑101       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  19:511503/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  7:78900/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  18:562843/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  19:105452/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  5:511503/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  5:105452/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  4:562843/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                                                       <  21:78900/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGGGTAAATGGCCTTTGG.GGTGATTGGCT.GGGCTTTGGC.ATTGCGCCCAAGGGCCTTacac                                                                                       >  1:32429/1‑101       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGGGTAAATGGCCTTTGG.GGTGATTGGCT.GGGCTTTGGC.ATTGCGCCCAAGGGCCTTacac                                                                                       >  15:32429/1‑101        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                                                      >  6:214165/1‑100        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGAT.GGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                                                      >  20:214165/1‑100                                                                   gTGAT.GACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                            <  26:688525/101‑1                                                                   gTGAT.GACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                            <  12:688525/101‑1                                                                                         aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac  <  20:20670/101‑2                                                                                         aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac  <  6:20670/101‑2                                                                                                 ||                                                                                                  GCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGAT.GGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGAT  >  NC_013198/1872997‑1873187 Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG