Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,274	C→T	L177L (CTG→CTA)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,274	0	C→T	100.0%	32.4	8	L177L (CTG→CTA)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (0/0):  total (4/4)

GCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCGGCGCGTTCAGAAGTCGGAGTGTAAGTGGCCTCAGACGTGAT  >  NC_013198/1873206‑1873307                                                                     |                                  gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  >  15:289620/1‑101 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  17:43682/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  >  1:289620/1‑101 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  21:1189025/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  >  21:292852/1‑101 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  3:43682/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  7:1189025/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  >  7:292852/1‑101                                                                     |                                  GCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCGGCGCGTTCAGAAGTCGGAGTGTAAGTGGCCTCAGACGTGAT  >  NC_013198/1873206‑1873307 Base quality scores:  ATCG  < 3 ≤  ATCG  < 30 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG