Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,152	C→G	S218T (AGC→ACC)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,152	0	C→G	100.0%	46.1	10	S218T (AGC→ACC)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/6):  ref base (0/0):  total (4/6)

GTGATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873063‑1873209                                                                                            |                                                          gTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                                                  <  12:688525/101‑1 gTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                                                  <  26:688525/101‑1                     aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac                        <  20:20670/101‑2                     aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac                        <  6:20670/101‑2                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  17:362316/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  20:692944/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  3:362316/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  6:692944/1‑100                                                 ttCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  15:471050/101‑1                                                 ttCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  1:471050/101‑1                                                                                            |                                                          GTGATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873063‑1873209 Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG