Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,110	C→T	S232N (AGT→AAT)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,110	0	C→T	100.0%	32.0	8	S232N (AGT→AAT)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (0/0):  total (4/4)

GTGATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGT  >  NC_013198/1873063‑1873195                                                |                                                                                        gTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                                    <  12:688525/101‑1 gTGATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGGGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAAcc                                    <  26:688525/101‑1                     aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac          <  20:20670/101‑2                     aTTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT...ATG...GTGAGCCAGCCCGGTTAGAAACCGGAGCAGAAGCGGCCTTGGGCGtgac          <  6:20670/101‑2                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc  >  17:362316/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc  >  20:692944/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc  >  3:362316/1‑100                                   ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc  >  6:692944/1‑100                                                |                                                                                        GTGATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGT  >  NC_013198/1873063‑1873195 Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG