Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,091	2 bp→CA	coding (713‑714/795 nt)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,091	0	T→C	100.0%	142.9	38	S238S (TCA→TCG)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/36):  ref base (0/2):  total (0/38)
*	NC_013198	1,873,092	0	G→A	100.0%	132.1	38	S238L (TCA→TTA)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/38):  ref base (0/0):  total (0/38)

GCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGG  >  NC_013198/1872997‑1873163                                                                                                ||                                                                          gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGGCTTGGGCGG.GATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                     >  16:89383/1‑101 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGGCTTGGGCGG.GATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                     >  1:89383/1‑101 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                     <  6:845145/101‑1 gCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTcc                                                                     <  21:845145/101‑1   tGGCGGACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  14:200532/101‑1   tGGCGGACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  29:200532/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  4:76013/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  13:787787/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  4:1027104/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  16:146457/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  16:904841/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  19:1027104/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  1:904841/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  19:76013/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  28:787787/101‑1   tGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCtt                                                                   <  1:146457/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                  <  24:266188/101‑1    ggCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTa                                                                  <  9:266188/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  28:838392/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  27:652615/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  25:929062/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  10:929062/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  13:838392/101‑1     gCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTac                                                                 <  12:652615/101‑1      cGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTaca                                                                <  22:654494/101‑1      cGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTaca                                                                <  7:654494/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  23:40384/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  19:920235/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  8:40384/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  18:1204742/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  3:1204742/101‑1       gAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacac                                                               <  4:920235/101‑1        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                              <  11:771627/101‑2        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                              <  23:801214/101‑2        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                              <  8:801214/101‑2        aaCGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCG.TGATGGCCTGGGCTT.GGCCATTGCGACCAAGGTCCTTacact                                                              <  26:771627/101‑2              ttATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGC.GTGATGGCC.CGCTCTTGGCCATTGCGCTTAAGCTCCTTACACGCAGACt                                                        <  4:1229565/101‑1              ttATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGC.GTGATGGCC.CGCTCTTGGCCATTGCGCTTAAGCTCCTTACACGCAGACt                                                        <  19:1229565/101‑1                                                                     gATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCgg  <  13:724536/101‑1                                                                     gATGACC.GGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCgg  <  28:724536/101‑1                                                                                                ||                                                                          GCTGGCGAACGCGTTATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTAGGCGGTGATGGCC.GGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGG  >  NC_013198/1872997‑1873163 Base quality scores:  ATCG  < 3 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG