Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,245	T→A	D187V (GAT→GTT)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,245	0	T→A	100.0%	17.3	6	D187V (GAT→GTT)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (0/0):  total (0/6)

GCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCGGCGCGTTCAGAAGTCGGAGTGTAAGTGGCCTCAGACGTGAT  >  NC_013198/1873206‑1873307                                        |                                                               gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  17:708253/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  18:273237/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  24:609387/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  2:708253/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  3:273237/101‑1 gcgcCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTT.ATGAGCGTGAACCGGCGCGCTTAGGGATCGGAGTGTAAGTGGCCTTGGGCGTGAt  <  9:609387/101‑1                                        |                                                               GCGCTCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGCGATCGCGTTTCAGAGCGTGAACCGGCGCGTTCAGAAGTCGGAGTGTAAGTGGCCTCAGACGTGAT  >  NC_013198/1873206‑1873307 Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG