Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,180	A→G	S209P (TCA→CCA)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,180	0	A→G	100.0%	41.9	10	S209P (TCA→CCA)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (0/0):  total (6/4)

GAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873092‑1873209                                                                                           |                              aaGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGAc                     >  11:247060/2‑101 aaGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGAc                     >  26:247060/2‑101      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                <  12:640023/101‑2      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  16:541764/1‑100      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  1:541764/1‑100      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  24:486295/1‑100      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                <  27:640023/101‑2      ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  9:486295/1‑100                    ttCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  23:45488/101‑1                    ttCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  8:45488/101‑1                                                                                           |                              GAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873092‑1873209 Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG