Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_013198	1,873,122	C→T	R228H (CGC→CAC)	LGG_01843	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	1,873,122	0	C→T	100.0%	18.9	12	R228H (CGC→CAC)	LGG_01843	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (2/2):  total (6/6)

GATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873065‑1873209                                                          |                                                                                          gATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCgg                                                <  13:724536/101‑1 gATGACCGGGCTTTGGTCATTGCGCCCAAGGTCCTTATGTGCAGGTTTCTGGGCTGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCgg                                                <  28:724536/101‑1                            aaGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGAc                     >  11:247060/2‑101                            aaGGTCCTTATGTGCAGGTTTCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGAc                     >  26:247060/2‑101                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                <  12:640023/101‑2                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  16:541764/1‑100                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  1:541764/1‑100                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  24:486295/1‑100                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                <  27:640023/101‑2                                 ccTTACACTCCGATCCCTGCGCCGGTGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCgggc                >  9:486295/1‑100                                               ttCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  23:45488/101‑1                                               ttCTGGGCTGGCGAACGCGTTATGGTGAGCGTGAACCAGCCCGGTTAGAAACCGGAGCATAAGTGGCCTTGGGCGTGATGACCGGGCTTTGGTCATTgcgc  <  8:45488/101‑1                                                          |                                                                                          GATGGCCGGGCTTTGGCCATTGCGGCTGAGGTCCTTACACGCAGACTTCTGCGCCGGCGAACGCGTT.T.ATGAGCGTGAACCGACGCGCTTAGAAACCGGAGCATAAGTGGCCTTGAGCGTGATGACCGGGTTTTGGTCATTGCGC  >  NC_013198/1873065‑1873209 Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG