Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_013198	508,609	0	G→T	100.0%	1.5	2	R133R (CGC→CGA)	is27	transposase IS5 family protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/0):  total (0/2)

GTAGCGTCTGTGATCAAGACACGGAGCTCATCATCTGGCAATAAGCTTTGAATTTGTTC.GCGCAAGGTATCACTGATGATATTTCGCACTAGTTCCGTTAT  >  NC_013198/508550‑508650                                                             |                                          gTAGCTACTGTGATCAAGACACGCATCTGCTCATGTGGAAATAAGCTTT.AATTTTTTCCTCGCAAGGTATCACTGATGATATTTCGCACTAGTTCCGTTAt  <  24:688578/101‑1 gTAGCTACTGTGATCAAGACACGCATCTGCTCATGTGGAAATAAGCTTT.AATTTTTTCCTCGCAAGGTATCACTGATGATATTTCGCACTAGTTCCGTTAt  <  9:688578/101‑1                                                             |                                          GTAGCGTCTGTGATCAAGACACGGAGCTCATCATCTGGCAATAAGCTTTGAATTTGTTC.GCGCAAGGTATCACTGATGATATTTCGCACTAGTTCCGTTAT  >  NC_013198/508550‑508650 Base quality scores:  ATCG  < 3 ≤  ATCG  < 9 ≤  ATCG  < 10 ≤  ATCG  < 25 ≤  ATCG  < 37 ≤  ATCG